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Sameer, Konda
- Evaluation of phyllosphere antagonistic bacteria on the management of Fusarium ear rot of maize caused by Fusarium verticillioides
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Authors
Affiliations
1 Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Thoothukudi District, Tamil Nadu, IN
1 Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Thoothukudi District, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 32, No 4 (2018), Pagination: 257-263Abstract
Fusarium verticillioides is an insidious fungal pathogen of maize associated with diseases such as ear rot and kernel rot. It attacks the phyllosphere region of the maize plant especially on inflorescence and cobs. Thus, this study was conducted to isolate potential phyllosphere colonizing antagonistic microbes for the management of Fusarium ear rot. Four phyllosphere colonizing antagonistic bacteria were found to be effective in suppression of the growth of Fusarium verticillioides. Based on 16S rDNA analysis, these bacterial isolates were identified as Pseudomonas aeruginosa isolate 1, P. aeruginosa isolate 2, Bacillus subtilis isolate and B. amyloliquefaciens. Among these four phyllosphere bacteria tested against F. verticillioides, the maximum inhibition of mycelial growth of F. verticillioides and production of volatile compounds was exhibited by P. aeruginosa isolate 1. Application of P. aeruginosa isolate -1, as seed treatment @ 10 g/kg of seeds, soil application @ 5 g/pot and foliar spray @ 0.2 per cent, recorded the minimum PDI with the maximum disease reduction over control. Thus, the present study showed that P. aeruginosa isolated from agricultural ecosystem could be a potential phyllosphere antagonistic bacterium for the management of maize ear rot disease.
Keywords
Fusarium verticillioides, Fusarium ear rot, Phyllosphere antagonistic bacteria, Pseudomonas aeruginosa, 16S rDNA Analysis.Full Text
References
- Andrews JH, Spear RN, Nordheim EV. 2002. Population biology of Aureobasidium pullulans on apple leaf surfaces. Can J Microbiol. 48: 500-513. https://doi.org/10.1139/w02-044 PMid:12166677
- Cavaglieri L, Orlando J, Rodríguez MI, Chulze S, Etcheverry M. 2005. Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize ischolar_main level. Res Microbiol. 156: 748-754. https://doi.org/10.1016/j.resmic.2005.03.001 PMid:15950130
- Dennis C, Webster J. 1971. Antagonistic properties of species groups of Trichoderma. III. Hyphal interactions. Trans Brit Mycol Soc. 57: 363-369. https://doi.org/10.1016/ S0007-1536(71)80050-5
- Durairaj K, Velmurugan P, Park JH, Chang WS, Park YJ, Senthilkumar P, Choi KM, Lee JH, Oh BT. 2017. Potential for plant biocontrol activity of isolated Pseudomonas aeruginosa and Bacillus stratosphericus strains against bacterial pathogens acting through both induced plant resistance and direct antagonism. FEMS Microbiol Lett. 364 (23) fnx225. https://doi.org/10.1093/femsle/fnx225 PMid:29069329
- Gerhardt P, Murray RGE, Costelow RN, Nexter EW, Wood WA, Dreig NP, Phillips GB. 1981. Manual of methods of general bacteriology. American Society of Microbiol. p. 400-450.
- Innerebner G, Knief C, Vorholt JA. 2011. Protection of Arabidopsis thaliana against leaf-pathogenic Pseudomonas syringae by Sphingomonas strains in a controlled model system. Appl Environ Microbiol. 77: 3202-s3210. https://doi.org/10.1128/AEM.00133-11 PMid:21421777 PMCid:PMC3126462
- Lorck H. 1948. Production of hydrocyanic acid by bacteria. Physiol Plant. 1: 142-146. https://doi.org/10.1111/j.1399-3054.1948.tb07118.x
- Monnier N, Furlan A, Botcazon C, Dahi A, Mongelard G, Cordelier S, Clément C, Dorey S, Sarazin C, Rippa S. 2018. Biotechnological potential of a rhizosphere Pseudomonas aeruginosa strain producing phenazine1-carboxylic acid and phenazine-1-carboxamide. Front Plant Sci. 9:1170. https://doi.org/10.3389/ fpls.2018.01170 PMid:30135699 PMCid:PMC6092566
- Nayaka SC, Sahankar ACU, Reddy MS, Niranjana SR, Prakash HS, Shetty HS, Mortensen CN. 2008. Control of Fusarium verticillioides, cause of ear rot of maize, by Pseudomonas fluorescens. Pest Manag Sci. 65: 769-775. https://doi.org/10.1002/ps.1751 PMid:19347968
- Nourozian J, Etebarian HR and Khodakaramian G. 2006. Biological control of Fusarium graminearum on wheat by antagonistic bacteria Songklanakarin. J Sci Technol. 28: 29-38.
- Payne GA. 1999. Ear and kernel rots. In: White, DG (Ed.). Compendium of Corn Diseases. Third Edition. American Phytopathological Society, St. Paul, MN, USA. 44–49. PMid:10216195
- Reid LM, Hamilton RI, Mather DE. 1996. Screening Maize for Resistance to Gibberella Ear Rot. Agriculture and Agri-Food. Canadian Technological Bulletin Publication, 196-5E.
- Schaad NW. 1992. Laboratory guide for identification of plant pathogenic bacteria. APS Press, St. Paul, Minnesota. P. 164.
- Schwyn B, Neilands JB. 1987. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 160: 47-56.https://doi.org/10.1016/0003-2697(87)90612-9
- Seely W, Vandemark J. 1981. Microbes in action. A laboratory manual of microbiology. The W.H. Freeman and Company Inc. (3rd edition) Sanfrancisco.
- Sreenivasa MY. 2006. Molecular detection of fumonisin producing Fusarium species of freshly harvested maize kernels using Polymerase Chain Reaction (PCR). Taiwania-Taipei 51(4): 251.
- Suslow TV, Schroth MN, Isaka M. 1982. Application of rapid method for gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathol. 72: 917-918. https://doi.org/10.1094/Phyto-72-917
- Tutte J. 1969. Plant Pathological methods. Fungi and bacteria. Burgess publishing company, U.S.A. 229.
- Vidhyasekaran P, Muthamilan M. 1995. Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis. 79: 782-786. https://doi.org/10.1094/PD-79-0782
- Visentin I, Tamietti G, Valentino D, Portis E, Karlovsky P, Moretti A, Cardinale F. 2009. The ITS region as a taxonomic discriminator between Fusarium verticillioides and Fusarium proliferatum. Mycol Res. 113: 1137-1145. https:// doi.org/10.1016/j.mycres.2009.07.011 PMid:19631741
- Uzair B, Kausar R, Bano SA, Fatima S, Badshah M, Habiba U, Fasim F. 2018. Isolation and molecular characterization of a model antagonistic Pseudomonas aeruginosa divulging In Vitro plant growth promoting characteristics. Biomed Res Int. 6147380 https://doi.org/10.1155/2018/6147380
- Vorholt JA. 2012. Microbial life in the phyllosphere. Nat Rev Microbiol. 10: 828-840. https://doi.org/10.1038/ nrmicro2910 PMid:23154261
- Zhou L, Jiang HX, Sun S, Yang DD, Jin KM, Zhang W, He YW. 2016. Biotechnological potential of a rhizosphere Pseudomonas aeruginosa strain producing phenazine1-carboxylic acid andphenazine-1-carboxamide. World J Microbiol Biotechnol. 32(3): 50. https://doi.org/10.1007/s11274-015-1987-y PMid:26873561